◆ in vitro evaluation of drug absorption, distribution, metabolism and excretion (ADME)
▶ Study on drug permeability and transport in vitro
■ permeability of drugs studied by Caco-2 or mdckii-mdr1 monolayer cell model
■ Caco-2, mdckii-mdr1 cells and specific inhibitors for the study of transporter substrates and inhibitors
▶ Study on metabolic stability
■ study on the stability of drugs in liver microsomes (human, mouse, dog, monkey, etc.), S9, cytoplasm or hepatocytes
■ study on the stability of drugs in plasma, serum or whole blood
▶ Identification of metabolites and analysis of metabolic pathway in vitro
▶ Evaluation of drug solubility and chemical stability
▶ Study and evaluation of pre formulation
◆ pharmacokinetic evaluation of animals in vivo
▶ Bioavailability of various animal species (IV, PO)
■ rats, mice, dogs, monkeys, etc
▶ Kinetic evaluation of different routes of administration
■ intravenous, oral, intraperitoneal, subcutaneous and intramuscular injection, etc
▶ Tissue distribution and drug concentration measurement of various biological samples
■ plasma, whole blood, urine, feces, bile and tissues of the whole body, etc
▶ Analysis of drug excretion pathway and identification of metabolites in BDC rats